Are there scenarios where you send additional antibodies beyond anti-centromere, Scl-70, and RNA polymerase III in suspected scleroderma patients?   

While these are the most common ANA subtypes in scleroderma, there are several other antibodies that are important. High titer ANA's with a speckled pattern may be U1 RNP and may be associated with overlap disease (MCTD). ANA's with a nucleolar pattern can be U3 RNP (fibrillarin), Th/To, or  PM...