Does biotin interfere with the measurement of other metabolites of the Vitamin D pathway outside of the 25-hydroxy Vitamin D assay?
Answer from: at Academic Institution
Biotin interferes with chemiluminescent assays. Assays using LCMS to measure 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D are not affected.