In drawing blood cultures from a central line to evaluate for CLABSI, do you advise drawing separate blood cultures from each port in case of dual or triple lumen line?   

You don't need to use the central line to draw those cultures. Using the line to draw blood can in it by itself pose risk of introducing a microorganisms. NHSN CLABSi definition does not call for blood culture to be done from a line. 

I recently reviewed the perspective article from OFID (Mermel & Rupp, PMID 38770214) that tried to address the nuances related to the diagnosis of CLABSI and provided some expert guidance. I strongly recommend reading this article.The authors begin the discussion by saying there is no practical ...

Although drawing blood culture from a central line is not recommended in general, the old IDSA guideline in 2009 mentioned "If a blood sample cannot be drawn from a peripheral vein, it is recommended that ⩾2 blood samples should be drawn through different catheter lumens (B-III)....